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PP2Cs-SAPK2 mediates ABI5 phosphorylation SAPK2, predicted to function as a serine/threonine kinase, demonstrated strong autophosphorylation activity, as confirmed by immunoblot analysis using anti-phospho-serine/threonine (anti-Ser/Thr) antibodies (Supplementary Fig

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Mice were then transcardially perfused with ice-cold PBS (Biological Industries) before spleens, deep cervical lymph nodes, Peyers patches, and mesenteric lymph nodes were excised and mashed with the plunger of a syringe against a 100 m pore size cell strainer (Corning)

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